Identification And Characterisation Of Amino Acids

Biochem
Identification and characterisation of aminic acids



INDEX:
Aim & flat coatp.
Methodologyp.
Results & Observationsp.
Calculations p.
Discussion & Conclusionp.
Referencesp.

INTRODUCTION & BACKGROUND
Aim:

To empathise and study the mechanism for identifying the amino acid constituents in an unfathomable dipeptide and determining which amino acid is on the C-terminal and which is on the N-terminal of the dipeptide.

understate:

The compound 1-fluoro-2,4-dinitrobenzene (FDNB) pits with free amino, glyoxaline, and phenolic groups at neutral to basic pH.s to yield the corresponding, colored dinitrophenyl (DNP) compounds. Thus, the N-amino group on alone amino acids, the ?-amino group on lysine, the hydroxy group on tyrosine, and the imidazole group on histidine of all free amino acids, oligopeptides, and polypeptides controvert to form DNP derivatives.

The reaction of FDNB with a peptide precedes by the attack of an dead ? amino group on the electron-deficient carbon atom next to the fluorine atom( see fig 1). For this reason the reaction resolve is buffered at about pH 9.0 with NaHCO3. The reaction proceeds with rout of HF, so that additional NaHCO3 may be required during the reaction.



A strong base is not used because OH- ions also react with FDNB to form phenol as a side product.
chassis 1.

If the DNP derivative of a peptide is hydrolyzed in acid and, epoch acidified, extracted with aether, all nonpolar (uncharged) entities will be extracted into the ether phase while all polar components will remain in the sedimentary phase. Thus, the nonpolar N-terminal DNP-amino acids and diDNP-amino acids (from N-terminal lysine, tyrosine. or histidine) will be removed into the ether phase because their carboxyl groups are protonated (i.e., uncharged). Exceptions to this are those amino acids that yield polar side chain residues in acid such(prenominal) as DNP-arginine and DNPcysteic acid. In contrast, any amino acids that are not...If you loss to get a full essay, order it on our website: Orderessay



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